Current clamp-based recordings of action potentials in human sensory neurons. The effect of novel drug candidates on human sensory neuron excitability can be assessed in these kind of experiments.

Cardiac Disease


Viable human heart

1.  Electrophysiology-based drug profiling (Atrial fibrillation, Ventricular arrhythmia)

     I. Sharp electrode recording of membrane potential                

     II. Ventricular and atrial tissue

2.  Myocardial tissue contractility (heart failure, ventricular hypertrophy)

3.  Cardiac mesenchymal stem cells  (heart failure, ventricular hypertrophy)

4.  Coronary artery disease

5.  Valvular disease


Pain


Adult human DRG neurons

1.  Imaging-based drug profiling

     I. Ligand-gated channels and receptors

     II. Cold-, warm-, mechano-activated channels and receptors

     III. Voltage gated channels (proprietary Electrical Field Stimulation technology)

     IV. In vitro human models for:

          i. Inflammatory pain

          ii. Neuropathic pain


2.  Electrophysiology-based drug profiling

     I. Voltage clamp  (voltage-, ligand-gated channels)

          i. IC50 determination; frequency-, voltage-dependence

     II. Current clamp

          i. Modulation of neuronal excitability (step or ramp current injection)

          ii. Modulation of response to sensitizing agents

          iii. In vitro human models

               i. Inflammatory pain

               ii. Neuropathic pain


3.  Inhibition of NGF signaling pathway

​     I.    Neurite outgrowth/pruning 

Therapeutic Areas

(858) 366-8608

Calcium imaging-based profiling of human sensory neurons responses to chemical and physical stimulations. This technology provides an efficient way to test the activity of new drug candidates on large populations of human neurons.

Voltage clamp-based recordings of voltage gated sodium channels current in human sensory neurons. This methodology allows the profiling of voltage gated channels expressed in human sensory neurons and the determiknation of the potency of novel drug candidates.

Itch


Adult human DRG neurons

1.  Imaging-based drug profiling

     I. Pruritogen-induced responses (histamine, serotonin, chloroquine etc.)

     II. Pruritogen-induced responses in the presence of pro-inflammatory agents

2.  Electrophysiology-based drug profiling

     I. Voltage clamp  (voltage-, ligand-gated channels)

          i. IC50 determination

     II. Current clamp

           i. Modulation of response to pruritogens and sensitizing agents